Angioplasty: Progenitor Cells for Amputation Prediction | Protocol Preview

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Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia - a 2 minute Preview of the Experimental Protocol

Juan Antonio Suárez-Cuenca, Eduardo Vera-Gómez, Alejandro Hernández-Patricio, Atzín Suá Ruíz-Hernández, Juan Ariel Gutiérrez-Buendía, Carlos Ramiro Zamora-Alemán, Alberto Melchor-López, Yasser Alberto Rizo-García, Oscar Antonio Lomán-Zúñiga, Ignacio Escotto-Sánchez, Juan Miguel Rodríguez-Trejo, Rebeca Pérez-Cabeza de Vaca, Mario Antonio Téllez-González, Paul Mondragón-Terán
Centro Médico Nacional 20 de Noviembre, ISSSTE, Experimental Metabolism and Clinical Research Laboratory, Clinical Research Department, Division of Biomedical Research; Centro Médico Nacional 20 de Noviembre, ISSSTE, Vascular Surgery and Angiology Department; Centro Médico Nacional 20 de Noviembre, ISSSTE, Regenerative Medicine and Tissue Engineering Laboratory; Coordination of Research;

Lower limb amputation may occur even after angioplasty of obstructed vessels in Critical Limb Ischemia (CLI). Mononuclear Progenitor Cells (MPCs) reflect vascular repair. The present protocol describes the quantification of MPCs from circulation close to angioplasty, and its relationship with endothelial dysfunction and prediction of lower limb amputation.

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