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How to easily set up a 3D time lapse acquisition

Автор: Leica Microsystems Tutorials

Загружено: 2022-08-19

Просмотров: 2189

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Learn in this microscopy tutorial: How to easily set up a 3D time lapse acquisition

More about Mica: https://fcld.ly/mica-yt-tut

Mica, the world’s first wholly integrated imaging Microhub, brings widefield imaging, confocal imaging and with AI-supported analysis seamlessly together. All united in one sample-protecting incubator environment.

All researchers, regardless of expertise, can now work in one easy-to use digital imaging platform, moving confidently from setup to beautifully visualized results, allowing true access for all.

At last, users can capture 4 x more data with 100% correlation in only one exposure, whether using widefield or confocal imaging. They can freely switch from widefield to confocal mode with just a simple click without ever moving the sample and explore unexpected paths with no constraints.

Mica also fully integrates everything a researcher needs for radically simplified workflows, using automation and AI to enable deeper understanding and a faster track to publication.

With Mica you have everything you need - all in one place - to supercharge fluorescence imaging workflows and get meaningful scientific results faster. Now you can focus on your science, not on figuring out your microscope.

#microscopy #tutorial #microhub

▬ Transcript ▬▬▬▬▬▬▬▬▬▬▬▬
How do you set-up a 3D time-lapse experiment?
Select the cell of interest, activate 3D Imaging and first of all it run the Z Stack Finder to specify the volume that you want to image.
Once that is done, activate Time lapse and specify the time-lapse experiment conditions.
Here we want to have our Z-Stack being recorded as fast as possible for a duration of about 2 minutes.
Click start.
This is our data set in 3D.

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Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Widely recognized for optical precision and innovative technology, the company is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning, and super-resolution microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopy.

How to easily set up a 3D time lapse acquisition

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