Generation: Induced Pluripotent Stem Cells By Reprogramming Human Fibroblasts l Protocol Preview

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Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set - a 2 minute Preview of the Experimental Protocol

Dongmei Wu, Brad Hamilton, Charles Martin, Yan Gao, Mike Ye, Shuyuan Yao
Stemgent, Research and Development;

We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific markers.

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