BRADFORD ASSAY: PROTEIN QUANTIFICATION

🎥 Bradford Assay for Protein Quantification | Step-by-Step Protocol & Tutorial
🔬 Welcome to our comprehensive tutorial on the Bradford Protein Assay, one of the most widely used methods for protein quantification in biochemical and molecular biology labs. Whether you're a student, researcher, or lab technician, this video will guide you through a detailed, step-by-step protocol to help you master the technique with confidence.
📌 In this video, you'll learn:

What the Bradford Assay is and how it works
Reagents and materials needed
How to prepare the Bradford reagent
Sample preparation and dilution
Creating a standard curve using BSA (Bovine Serum Albumin)
Measuring absorbance using a spectrophotometer
Calculating protein concentration from your data
Troubleshooting common issues

✅ Perfect for:

Undergraduate and graduate students
Lab instructors and demonstrators
Researchers in biochemistry, molecular biology, and biotechnology

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📚 Related Topics:
Protein assays comparison, Lowry vs Bradford, colorimetric assays, lab protocol tutorials, protein biochemistry
🔗 Chapters:
00:00 Introduction
01:15 Principle of Bradford Assay
03:00 Materials & Reagents
05:20 Sample Preparation
07:10 Standard Curve Setup
09:00 Spectrophotometer Reading
10:30 Data Analysis
12:00 Troubleshooting Tips
13:30 Final Thoughts
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