UC Enuity Ultramicrotome – Trim your target with the help of fluorescence

Trimming a block face is a time-intensive task and takes a certain level of training. In addition, sometimes the sample is buried inside of the resin and the target not visible increasing the risk to lose the sample during trimming. Using fluorescence imaging allows to visualize a fluorescent sample inside the resin. UC Enuity makes use of the fluorescence and enables a precise automatic trimming. Just visualize the target, define the boundaries, and walk away!
Many thanks to Dr. Felix Gaedke, CECAD Cologne, for supporting sample preparation and sectioning.

00:00 – Unveiling the UC Enuity Ultramicrotome
00:29 – Fluorescence imaging setup
00:37 – Loading of sample, knife, MesaCut mirror and shielding
00:55 – Selection of excitation filter depending on sample labeling
01:29 – Define the boundaries
01:33 – Trimming in process
01:43 – Automatic turn of sample by 90°
01:49 - Result of the automatic trimming process under fluorescence (fern spore)

#ultramicrotomy #fluorescence #trimming #Ultramicrotome
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The UC Enuity Ultramicrotome: https://www.leica-microsystems.com/pr...

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Leica Microsystems develops and manufactures microscopes and scientific instruments for the analysis of microstructures and nanostructures. Ever since the company started as a family business in the nineteenth century, its instruments have been widely recognized for their optical precision and innovative technology. It is one of the market leaders in compound and stereo microscopy, digital microscopy, confocal laser scanning microscopy with related imaging systems, electron microscopy sample preparation, and surgical microscopes.