ELISpot tutorial: step-by-step full assay protocol

Learn how to perform an ELISpot assay with Mabtech in this step-by-step tutorial video. Follow along as we go through the entire protocol with a number of helpful tips along the way.

0:00 Day 1 - activating and coating ELISpot plate
0:05 Materials for activating ELISpot plate
0:47 Activating PVDF membrane of the ELISpot plate
3:17 Prepare coating antibody
5:06 Day 2 - conditioning ELISpot plate and adding stimuli plus cells (start here if using pre-coated plates from ELISpot Plus and ELISpot Pro kit formats)
6:31 Adding stimuli - prepare peptide pool and addition
8:24 Adding cells
9:25 Day 3 - detection and spot development
10:25 Preparing detection antibody
11:33 Filtering and adding detection antibody
12:25 Prepare streptavidin-enzyme conjugate
13:49 Adding streptavidin-enzyme conjugate
14:46 Developing plate - filtering and adding substrate
16:50 Stop spot development and dry the plate
17:10 Counting spots with IRIS or ASTOR

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