F*cking Westernblot - Remix by Chacal

(Verse 1)
In the lab, under the fluorescent lights,
With pipettes in hand, we start our nightly fights.
Proteins to measure, our hopes held high,
But deep down we know, it's all a lie.

(Chorus)
Western blot, oh, Western blot,
Why do you torment us, make us distraught?
From transfer to detection, it's all just a mess,
In this lab life, we're under duress.

(Verse 2)
First, we lyse our cells with care,
Protease inhibitors, in buffer we prepare.
Spin down the lysate, get that supernatant clear,
Load it in the wells, with trembling and fear.

(Chorus)
Western blot, oh, Western blot,
Why do you torment us, make us distraught?
From transfer to detection, it's all just a mess,
In this lab life, we're under duress.

(Bridge)
Run the gel, proteins separate,
In SDS-PAGE, they migrate.
Transfer to membrane, PVDF or nitro,
But will it work? We never know.

(Verse 3)
Block with milk, an hour or two,
Primary antibody, we hope it’s true.
Wash it away, add the secondary,
But the signal’s weak, our hearts grow wary.

(Chorus)
Western blot, oh, Western blot,
Why do you torment us, make us distraught?
From transfer to detection, it's all just a mess,
In this lab life, we're under duress.

(Bridge)
Expose the film, develop with dread,
What will we see? A smear instead.
No bands in sight, or nonspecific stains,
Our hard work, it seems, was all in vain.

(Verse 4)
Reagents fresh, repeat once more,
Same results, our spirits sore.
Colleagues console us, say it’s part of the game,
But deep down we know, it’s always the same.

(Chorus)
Western blot, oh, Western blot,
Why do you torment us, make us distraught?
From transfer to detection, it's all just a mess,
In this lab life, we're under duress.

(Verse 5)
Reagents fresh, repeat once more,
Same results, our spirits sore.
Colleagues console us, say it’s part of the game,
But deep down we know, it’s always the same

(Outro)
In the end, we pack up, tears in our eyes,
Another failed blot, no surprise.
In biology labs, we all share this plight,
Western blot blues, every single night.