Drosophila melanogaster: Make a cross | | UPV

Título: Drosophila melanogaster: Make a cross

Descripción: This learning object describes the main characteristics of Drosophila melanogaster for making a cross and the steps to mate them in the laboratory are listed. Leiva Brondo, M. (2019). Drosophila melanogaster: Make a cross. http://hdl.handle.net/10251/120967

Descripción automática: In this video, the presenter introduces the topic of crossbreeding Drosophila melanogaster, commonly known as fruit flies. The main goal is to explain the principal characteristics of the species and provide a guide on how to perform genetic crosses.

The presenter reviews the anatomy of the fruit fly, distinguishing different body parts such as the head, thorax, wings, and legs. The importance of distinguishing the fruit fly from the Mediterranean fruit fly is emphasized due to the latter's pest status on fruit trees. The presenter outlines the fruit fly's life cycle, passing through stages from embryo to larva, pupa, and finally, adult.

The breeding process involves selecting virgin females and mates, crucial for ensuring the purity of the genetic cross. The presenter details how to identify virgin females, noting they are not fertile until around 12 hours after emerging from the pupa. Identification of males is aided by the presence of sex combs on their forelegs, which are not found on females.

The presenter explains the genetic crossing process, using schemes to breed different generations (F1, F2, and backcrosses) and describes how to maintain controlled breeding conditions. Key to successful breeding is ensuring that the females mate with the chosen males and not others, as females can store sperm from multiple males.

The video concludes with a discussion on the control and study of traits across generations, emphasizing the use of virgin females for accurate crosses. The presenter also provides references for further reading and thanks the viewers for their attention.

Autor/a: Leiva Brondo Miguel



Universitat Politècnica de València UPV: https://www.upv.es
Más vídeos en:    / valenciaupv  
Accede a nuestros MOOC: https://upvx.es

#Model organism #Reproductive cycle #Developmental stages #Vinegar fly #Crop #GENETICA #2409 - Genética