How to Interpolate a Standard Curve in GraphPad Prism | ELISA Analysis, Standard Curve Fitting

How to Interpolate a Standard Curve in GraphPad Prism | ELISA Analysis, Standard Curve Fitting

Learn how to find unknown concentrations from a standard curve using GraphPad Prism. Ideal for beginners in ELISA analysis, pharmacokinetics, and biomedical research, this video covers:

✅ How to enter standard curve data (concentration vs absorbance)
✅ Fitting the curve using 4-parameter logistic (4PL) or linear regression
✅ Inputting unknown absorbance values
✅ Automatically interpolating concentrations of unknown samples
✅ Exporting results and customizing your graph for publication

📊 Tools used:
✔ Microsoft Excel: Statistical analysis
✔ GraphPad Prism (https://www.graphpad.com/free-trial)

📚 Disclaimer:
This video is for educational and informational purposes only. Always consult appropriate references and verify your results independently. We do not guarantee the accuracy or suitability of this method for all datasets or applications. Use your own discretion and judgment when applying these techniques.

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