Complete MTT Assay Analysis | How to Calculate IC50, % Cell Viability, Dose-response curve | Prism

Complete MTT Assay Analysis | How to calculate IC50, % Cell Viability, Dose-response curve | GraphPad Prism

In GraphPad Prism How to calculate IC50 value, % Cell viability, How to make dose-response curve/graph, How to plot histogram and sigmoidal curve. A step-by-step complete tutorial on MTT assay analysis using GraphPad Prism and Graph representation.

🔬 In this tutorial, you will learn:
✅ How to enter and structure your data for MTT assay analysis
✅ How to process and analyze cytotoxicity data
✅ How to calculate % Cell viability, SEM, SD, %RSD, 95% confidence interval
✅ How to plot sigmoidal curve and histogram for dose-response curve
✅ Customizing and formatting graphs in GraphPad Prism

📊 Tools used:
✔ Microsoft Excel: Statistical analysis
✔ GraphPad Prism (https://www.graphpad.com/free-trial)

📚 Disclaimer:
This video is for educational and informational purposes only. Always consult appropriate references and verify your results independently. We do not guarantee the accuracy or suitability of this method for all datasets or applications. Use your own discretion and judgment when applying these techniques.

🔔 Don’t forget to subscribe for more tutorials on GraphPad Prism, MTT assays, IC₅₀ calculations, and Python-based scientific analysis.

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