Decalcification in Histopathology - A complete Guide

Decalcification in Histopathology - A complete Guide

Discover the complete guide to bone decalcification in histopathology! This video explains everything you need to know about decalcification techniques, including acid decalcification (hydrochloric acid, nitric acid, formic acid), EDTA chelation, ion-exchange resin methods, and electrolytic decalcification. Learn how each method affects tissue morphology, nuclear staining, and overall histology quality, and get tips for proper endpoint determination using mechanical, chemical, and radiographic methods.
We also cover post-decalcification steps, including washing, neutralization, trimming, and troubleshooting common problems like bone dust, underdecalcification, and overdecalcification. Understand the importance of choosing the right decalcifying agent, monitoring time, and preserving tissue for optimal staining.
Ideal for histopathology students, laboratory technicians, and histology professionals, this video ensures you achieve high-quality bone histology, accurate staining, and reliable diagnostic results.

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