Confocal Imaging: Operating Inverted Zeiss LSM 880 Microscope using Zen Black 2.3 SP1 app.

Imaging parameters setting for confocal imaging
00:05 - Starting "Zen Black" app
00:19 - "Start System" wait for initialization to complete( takes about 40s)
00:50 - Power on lasers (01:07 to be precise)
Locating your sample
01:46 - Locate tab
01:56 - Selecting/On/Of filters
02:27 - Placing slide on the stage
04:55 - Changing Reflected light level
Imaging setup for Simultaneous scan for 3 Channel Imaging (Change track every line)
05:40 - Setting tracks
09:05 - Acquisition mode
09:16 - Select frame size, 09:23 - speed, 09:33 Averaging, 09:40 - Bit Depth, 09:45 - Scan
direction
09:57 - Setting Pin hole size to 1 AU (10:09 to be precise)

10:24 - Setting Laser power and Detector sensitivity
14:16 - Taking an Image
14:46 - Saving an Image

16:21 - Using smart setup to set up light paths

22:25 - Using pre-set imaging parameters and setting laser power and detector sensitivity as per need

26:17 - Using settings from previously taken image (Reuse)

29:14 - Imaging with Z-Stacks

31:53 - Maximum intensity projection of Z-stacks

33:50 - Tile-Scan Imaging
36:15 - Turning off Lasers and closing Zen Black App