Secret of Dynamic Light Scattering (DLS) for particle size analysis

Dynamic Light Scattering (DLS) is a mature and advanced technique in characterizing size and size distribution of particles dispersed in suspension, specifically in the nanometer and sub-micron range.

A warm welcome to the Bettersize Lab! Watch Bettersize's application engineer Shelly Zhang gives an introduction to Dynamic Light Scattering (DLS) for particle size analysis with the magnitude of 1-nanometer to10-micrometers.

The scattered light intensity depends on the particle's intrinsic physical properties such as size and molecular weight. The scattered light intensity is not a constant value; it fluctuates over time due to the random walk of particles that are undergoing Brownian motion. The fluctuations in scattered light intensity with time allow us to calculate the diffusion coefficient through the autocorrelation function analysis. Then using the Stokes-Einstein equation, the particle size distribution can be calculated from the diffusion coefficient. This technique is called dynamic light scattering, abbreviated as DLS.

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0:00 - Start
0:37 - Theory of DLS​
6:20 - Optical Setup
17:30​ - Sample preparation
24:02 - Result interpretation
27:19 Summary