Raman mapping with the Horiba LabRAM Aramis Raman microscope and LabSpec 5

This video will teach you how to use LabSpec 5 software to acquire hyperspectral Raman or photoluminescence maps with the Horiba LabRAM Aramis Raman microscope at the Molecular Foundry. This basic tutorial covers:

0. Overview of Raman mapping steps - 0:49
1. Initial microscope setup - 1:25 (see also    • How to use the Horiba LabRAM Aramis Raman ...  )
2. Defining your region of interest - 1:42
3. Setting map acquisition properties - 2:58
4. Setting and confirming spectral acquisition parameters - 4:49
5. Acquiring and visualizing maps - 6:41
Overlaying maps on bright field images - 8:13
Assigning map colors/intensities to spectral windows/ROI's - 9:45
Adjusting brightness/contrast of maps - 11:22 & 14:16
The Map Analysis button (activating map windows/ROI's/basline subtraction) 12:09
6. Saving hyperspectral data 15:08
7. Troubleshooting & Tips 16:39

Additional resources:

Standard operation of the LabRAM Raman microscope
   • How to use the Horiba LabRAM Aramis Raman ...  

Online PDF manual (LBNL users only)
https://sites.google.com/lbl.gov/rama...

Wavelength calibration of the LabRAM spectrometer
   • Wavelength calibration for the Horiba LabR...  

Using the NIR detector of the LabRAM microscope
   • How to use the NIR detector on the Horiba ...  

The Molecular Foundry is a Department of Energy nanoscience user facility in Berkeley, California, USA. The Horiba Raman microscope and many other instruments are available to scientists through the Molecular Foundry User Program. For more information, visit http://foundry.lbl.gov