SGD On Hay's Sulfur Test for Bile Salts: Biochemistry Practical Demonstration | MBBS

In this Biochemistry practical session at Santiniketan Medical College & Hospital, we demonstrate the Hay's Sulfur Test used for detecting Bile Salts in a urine sample. This video is an essential guide for 1st-year MBBS students (2025-2026 Batch) and BMLT/DMLT students preparing for their laboratory examinations and "OSCE" assessments.

Key Concepts Covered:

Bile Salts Identification: Identifying Sodium and Potassium salts of Glycocholate and Taurocholate [18:11].

Principle of the Test: How Bile Salts reduce surface tension to cause sulfur powder to sink [22:30].

Practical Demonstration: Step-by-step procedure of sprinkling sulfur powder on a 2/3 filled test tube [14:38].

Clinical Significance: Correlation between positive results and Obstructive Jaundice [40:28].

Comparative Review: Brief discussions on Benedict's Test, Heat Coagulation, and Rothera's Test [23:53].

Timestamp Breakdown:

[00:48] Reviewing the Heat Coagulation Test Principle & Denaturation.

[04:31] Introduction to the Hay's Sulfur Test.

[09:00] Why do we use Sulfur Powder? (Inertness to water and visibility).

[11:09] Overview of Qualitative Urine Analysis (Sugar, Protein, Ketones, Blood).

[12:43] The role of Distilled Water as a Control in lab experiments.

[15:22] Demonstration: Observing sulfur powder sink (Positive result).

[18:11] Naming the Bile Salts: Sodium/Potassium Glycocholate & Taurocholate.

[23:24] Gerhardt's Test vs. Rothera's Test for Ketone bodies.

[30:52] Purpose of Acetic Acid in Heat Coagulation tests.

[35:47] Difference between Macroscopic and Microscopic Hematuria.

[43:23] False Positives in Benedict's Test: Ascorbic acid and Salicylates.

[47:40] Why the Benzidine Test is discontinued (Carcinogenic properties).


SANTINIKETAN MEDICAL COLLEGE & HOSPITAL
Real Classroom Demonstration/Lecture
On: BIOCHEMISTRY by Dr. Karan Pal Singh
(MBBS Batch: 2025-26)
Conducted on: 15-01-26