Educator's Guide to Nanopore—16s: DNA extraction

DNA extraction: Column-based DNA purification using a Norgen microbiome kit.

00:00 Introduction
03:00 Sample lysis
14:54 Transfer and addition of binding buffers
25:14 Adding sample to columns
34:02 Washing columns
37:34 Drying columns and elution

This guide is an educator-focused introduction to Nanopore DNA sequencing. Its primary goal is to share resources and approaches that make it easier to use Nanopore in the classroom, especially (but not exclusively) for course-based undergraduate research experiences (CUREs). In a series of videos, we will explain the basics of Nanopore sequencing as we share demonstration experiments.

Full protocol available at: https://nanopore4edu.org/latest/annot...