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Tissue Culture Series #1: How to Thaw Cells with High Efficiency
Автор: MilliporeSigma
Загружено: 11 февр. 2022 г.
Просмотров: 18 319 просмотров
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Tutorial demonstrating best practices for starting a fresh cell culture from frozen stocks, including cell thawing tips and tricks to maximize survival.
• Ice crystals and thaw time
• How to avoid osmotic shock
• Cryoprotectant removal and media replacement
• Thawing stem cells and primary cells
• Methods for resuspending cells
• Seeding density considerations
• Troubleshooting
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