L929 mouse fibroblast undergoes non-mitotic cell division | Cell budding | HoloMonitor®

This video shows a large, multin-ucleated L929 mouse fibroblast undergoing non-mitotic cell division (cell budding). It was captured completely label-free with HoloMonitor® and the colors were digitally added afterward.

Fibroblasts are the most common cells of the connective tissue, and under normal conditions they are mononuclear. However, there are two mechanisms by which they can acquire several nuclei; by fusion of several fibroblasts into one or by nuclear division without subsequent cell division. Multinucleated fibroblasts are usually related to fibroblastic pathologies such as fibrosis and cancer.

Cell budding is a form of non-mitotic cell division where a part of the cell membrane forms a small bud. As the bud grows, a nucleus and other cell organelles are transferred into it. Eventually, it detaches from the mother cell and forms a much smaller daughter cell. This way of multiplying is common for baker's yeast cells, Saccharomyces cerevisiae.


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