The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!

Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged?

Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.

In this video, we'll outline the steps/protocol for packaging lentiviruses, including:

• Considerations before you start [1:02]
• Basic packaging protocol [01:33]
• Storing your lentiviruses [4:19]
• Infection test experiment [4:56]
• Additional tips and tricks for lentivirus production

After watching this video, you’ll be well on your way to becoming a lentivirus production champion!

[4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use.

You can read more about how to package lentiviruses and get a hard copy of the protocol here:
➜ https://info.abmgood.com/lentivirus-s...

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Background music credit: "Back To Portland" by TrackTribe