How to prepare potato dextrose agar (PDA) using fresh potatoes

How to prepare potato dextrose agar (PDA) using fresh potatoes

Potato Dextrose Agar is a frequently used microbial growth media for cultivation of molds, yeasts and other fungi. It is made up of potato infusion and dextrose .The potato infusion and dextrose as a carbohydrate source support the luxuriant growth of fungi and bacteria and is observed to encourage mold sporulation and pigment production in certain dermatophytes. However, the media may be supplemented with selective agents such as acids (e.g. tartaric acid) or antibiotics (e.g. chloramphenicol, or chlortetracycline) to inhibit the growth of bacteria that can impede the yeasts and mold. Agar acts as a solidifying agent.

Applications
Potato Dextrose Agar (PDA) is commonly used in the dairy industry for detecting the presence of yeasts and molds in product samples. For this purpose, the addition of tartaric acid is recommended.
PDA supplemented with chlortetracycline is recommended for the microbial enumeration of yeast and mold from cosmetics.
PDA supplemented with Chloramphenicol is recommended for the selective cultivation of fungi from mixed samples.
PDA is used for isolation and enumeration of yeasts and molds in clinical samples.
Stock cultures of certain dermatophytes can be maintained on PDA media.
PDA is also used for stimulating sporulation and differentiating typical varieties of dermatophytes based on their pigment production.

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