Protein Purification

Protein Purification aims to isolate a single type of protein from a biological tissue or culture. This video explains the most common methods applied to purify proteins, which are:

Precipitation methods, filtration methods, electrophoresis and chromatographic methods.

It is important to have an overview of these techniques in order to choose which method/methods suit the project best. Factors that can influence such a decision are: the desired level of purity, the accessibility of required equipment and the characteristics of the target protein. In practice multiple purification techniques are often combined.

Time Code:
00:00 – Intro
00:20 – Content
00:49 – What is protein purification?
02:20 – Separate your protein from a biological tissue
02:53 – Choosing the right purification techniques
03:40 – Precipitation methods
03:58 – Salting out
05:10 – Chromatography
06:08 – Affinity chromatography
07:25 – Ion Exchange Chromatography
08:07 – Hydrophobic Interaction Chromatography
09:08 – Size Exclusion Chromatography
10:20 – Filtration Methods (Dialysis)
11:12 – Electrophoresis
12:08 – Native PAGE
13:29 – Outro

At Cube Biotech we are experts in the purification of protein with over 40 years of experience. We offer a wide range of protein purification products:

https://cube-biotech.com/products/pro...

Furthermore, we offer custom protein purification:

https://cube-biotech.com/protein-serv...

For general inquiries about our company check out:

https://cube-biotech.com/

For more detailed information about protein purification techniques check out:
Liu, S., Li, Z., Yu, B., Wang, S., Shen, Y., & Cong, H. (2020). Recent advances on protein separation and purification methods. Advances in colloid and interface science, 284, 102254. https://doi.org/10.1016/j.cis.2020.10...