Culture media (1): Mannitol salt agar media
Автор: Microstudy (Microbiology lectures)
Загружено: 2024-04-14
Просмотров: 680
Microstudy/Dr Ahmed Talaat
Medical microbiology
قناة متخصصه لشرح مادة الميكروبيولوجي والمناعة لجميع طلبة الكليات العملية
Culture media (1) : Mannitol Salt Agar
Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology. It encourages the growth of staphylococci while inhibiting the growth of others
It contains a high concentration (about 7.5–10%) of salt (NaCl) which is inhibitory to most bacteria - making MSA selective against most Gram-negative and selective for some Gram-positive bacteria (Staphylococcus, and Enterococcus) that tolerate high salt concentrations. It is also a differential medium for mannitol-fermenting staphylococci, containing mannitol and the indicator phenol red, a pH indicator for detecting acid produced by mannitol-fermenting staphylococci. Staphylococcus aureus produces yellow colonies with yellow zones, whereas other coagulase-negative staphylococci produce red colonies with no colour change to the medium
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