Hematoxylin and eosin staining procedure, H & E staining steps, H&E staining overview

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Hematoxylin and eosin (H&E) staining is a technique used to analyze tissue sections. It involves staining cell nuclei blue-black and cytoplasm pink-red.
Steps
Place tissue sections in a staining rack
Stain with filtered Harris Hematoxylin for 10 seconds
Rinse with tap water until clear
Stain with Eosin for about 30 seconds
Rinse with tap water until clear
Dehydrate with alcohol solutions (50%, 70%, 80%, 95% x 2, 100% x 2)
Clear with xylene
Mount with a coverslip and mounting medium
How it works
Hematoxylin stains cell nuclei purple-blue
Eosin stains cytoplasm and other tissues pink-red
The staining results depend on how well the tissue was processed
Uses
H&E staining is used to identify different types of cells and tissues
It helps diagnose diseases, such as cancer
It provides information about the shape, structure, and pattern of cells in a tissue sample
Other considerations
The staining results can vary based on the reviewer's experience
The staining process can take longer for paraffin-embedded sections than for frozen sections.