Lecture On Direct & Indirect Bilirubin Estimation: A Biochemistry Guide | MBBS | TSMC

This biochemistry lecture explains the estimation of direct and indirect bilirubin, starting with the breakdown of red blood cells (RBCs) in the spleen. The video details how hemoglobin from destroyed RBCs is converted to bilirubin, which is initially water-insoluble and transported by albumin. In the liver, bilirubin is conjugated with glucuronic acid, becoming water-soluble and eventually excreted.

The lecture emphasizes the clinical relevance of measuring direct (conjugated) and indirect (unconjugated) bilirubin levels. Elevated unconjugated bilirubin may indicate pre-hepatic issues like increased RBC destruction, while elevated levels of both types can suggest liver pathology. A high conjugated bilirubin level with normal unconjugated bilirubin may point to post-hepatic obstruction.

The video explains the practical procedure for direct bilirubin estimation, which involves a blank and a test, using a direct bilirubin reagent and a nitrite reagent. The reaction is measured at 578 nm, and the direct bilirubin concentration is calculated.

Note: The video does not provide specific normal reference ranges for direct and indirect bilirubin. It mentions that these values were discussed in a previous session. Please refer to your notes from that session for this information.



TRIPURA SANTINIKETAN MEDICAL COLLEGE
Real Class room Demonstration/Lecture On: (BIOCHEMISTRY) DIRECT BILIRUBIN ESTIMATION AND INDIRECT BILIRUBIN ESTIMATION
BY- DR. KARAN PAL SINGH
(MBBS Batch: 2024- 2025)
Conducted on:- 14-05-25