BioED webinar 21 - Arunas Stirke - PDMS-free microfluidic chips fabrication and utilization...

Arunas Stirke - Postdoctoral Researcher at Institute of Solid State Physics University of Latvia and FTMC in Lithuania - "PDMS-free microfluidic chips fabrication and utilization for protein treatment by PEF"
Abstract
The main goal of the research application is developing the microfluidic chip with multiple detection modules for evaluation of nsPEF (nanosecond duration pulsed electric field) induced gene expression suitable for melanoma gene therapy and treatment of protein by PEF. To handle the solution in a small chambers and treat by PEF at the same time is still challenging.
Dr. Arunas Stirke leads a young and interdisciplinary group of scientists investigating pulsed electric field effects on intracellular signaling and biological nanostructures (cell walls, plasma membrane) to develop innovative technologies and prototypes for modern food processing and healthcare.The main activities:To study the effect of electrical pulses on the plasma membrane permeability of cells (bacteria, yeast, algae as well as mammalian): a) to study the effect of plasma membrane permeability on the viability of wall-containing microorganisms; (b) to study the link between plasma membrane and cell-wall permeabilization and to search for selective test methods for this purpose; c) to investigate the mechanism of recovery of the permeabilized wall; (d) to develop and implement of electroporation-based technologies for the control of harmful micro-organisms;
To study the effect of short (nanosecond) electrical pulses on intracellular signals: a) to study the effect of plasma membrane permeability on the programmable death (apoptosis) of wall-containing microorganisms; (b) to investigate the mechanism of apoptosis of microorganisms induced by electrical and magnetic pulses; (c) to engineer gene expression promotors for controlled induction by electrical pulses;
To investigate the effect of abiotic factors such as light, temperature and electric field on cellular signals: a) to investigate the effects of nanostructured surfaces on thermal- and opto-permeabilization of cells; (b) to investigate cryoprotection mechanisms for cells and tissue; (c) to develop and apply new optically transparent polymeric materials for photodynamic therapy; d) to develop organ-on-a-chip devices to investigate electrically induced intercellular signals.
https://www.ftmc.lt/bioelectrics-labo...
https://www.ufe.cz/en/team/bioelectro...
https://www.ursi.org/homepage.php
https://www.ieee.org/