Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing.

Next Generation Sequencing (NGS) is used to sequence both DNA and RNA. Billions of DNA strands get sequenced simultaneously using NGS. Whereas with Sanger Sequencing, only one strand is sequenced at a time.

While the Human Genome Project took over 30 years to sequence the human genome for the first time. Now with Next Generation Sequencing, a whole human genome can be sequenced in just one day.

This video describes the library preparation process, cluster generation, the sequencing reaction and filtering and alignment of the sequencing data. This video focuses on the primary sequencing method used by Illumina, Sequencing by Synthesis (SBS).

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CHAPTERS:
00:00 From the Human Genome Project to NGS
00:39 NGS vs Sanger Sequencing
01:01 The Basic Principle of NGS
01:23 DNA and RNA Purification and QC
01:38 Library Preparation - The First Step of NGS
02:32 Sequencing by Synthesis and The Sequencing Reaction
03:19 Cluster Generation From the Library Fragment
03:59 Sequencing of the Forward Strand
04:42 The First Index is Read
04:55 The Second Index is Read
05:15 Sequencing of the Reverse Strand
05:25 Filtering and Mapping of the Reads
05:47 Demultiplexing and Mapping to the Reference
06:19 What is Read Depth in NGS?
06:49 How is NGS being used?
07:01 What Types of NGS Applications Are There?


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