Science of CRISPR I: Introduction to CRISPR-Cas9

How CRISPR-Cas9 Edits Human DNA: Step-by-Step Breakdown (Part 1 of a 4 part series)

CRISPR-Cas9 is one of the most powerful tools in modern science—and in this episode, we explain exactly how it works.

Whether you're a student, researcher, or just curious about gene editing, this video walks you through the full mechanism of CRISPR-Cas9, from DNA targeting to genetic modification.

🔍 In this episode, we cover:

What is CRISPR-Cas9 and how does it work?

How guide RNA and Cas9 find and cut specific DNA sequences

The difference between NHEJ (non-homologous end joining) and HDR (homology-directed repair)

What makes CRISPR precise—and where it can go wrong

How scientists use nickases for safer genome editing

Real-world delivery systems: how CRISPR gets inside human cells

⚡ Why it matters:
This is Part 1 of our 4-part series on CRISPR in living humans—including a U.S. breakthrough and a controversial Chinese experiment. Before we get into the ethical firestorms, we’re giving you a rock-solid foundation in the science.

👨‍🔬 Perfect for:

Biology and genetics students

Science enthusiasts

Anyone Googling "how does CRISPR-Cas9 work?"

📌 Subscribe now for upcoming episodes on:

Real-world gene editing in humans

The ethical debate surrounding CRISPR babies

The future of genome engineering

📺 Watch now and master the science behind gene editing before it edits us.

#CRISPR #GeneEditing #CRISPRCas9 #DNATechnology #GenomeEditing #Biotechnology #Genetics #ScienceExplained #HDRvsNHEJ #MolecularBiology #Nickase #Cas9Mechanism #GeneTherapy #CRISPRHumanTrials

Publications:

Jiang, Fuguo, and Jennifer A. Doudna. "CRISPR–Cas9 structures and mechanisms." Annual review of biophysics 46.1 (2017): 505-529.

Zhang, Yi-ran, Tai-lang Yin, and Li-quan Zhou. "CRISPR/Cas9 technology: applications in oocytes and early embryos." Journal of Translational Medicine 21.1 (2023): 746.

Maruyama, Takeshi, et al. "Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining." Nature biotechnology 33.5 (2015): 538-542.

Aljabali, Alaa AA, Mohamed El-Tanani, and Murtaza M. Tambuwala. "Principles of CRISPR-Cas9 technology: Advancements in genome editing and emerging trends in drug delivery." Journal of Drug Delivery Science and Technology 92 (2024): 105338.


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Time Stamps
0:00 - 0:40 - Introduction
0:41 - 1:49 - What are CRISPR and Cas9?
1:50 - 2:55 - How CRISPR-Cas9 Detects and Cuts
2:56 - 4:51 - Gene Editing via Repair
4:52 - 6:12 - Innovations in CRISPR-Cas9: Nickase and Adenoviruses.
6:13 - 8:22 - Outro