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Recombinant DNA technology ( Genetic engineering)
Автор: Biochemistry by Dr Rajesh Jambhulkar
Загружено: 2019-05-26
Просмотров: 376549
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Definition
manipulation of genetic material (DNA) to achieve a desired goal in a predetermined way.
Steps involved 6
1. Isolation of desired piece of DNA
2. Isolation of vector
3. formation of chimeric DNA
4. Introduction to host
5. multiplication and selection of cell
6. expression of gene to produce desired product
Tools of RDT
1. Enzymes
2. Vectors
3. Hosts
4. Gene transfer techniques
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