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Advanced Unmixing in SpectroFlo (Cytek Aurora)
Автор: UChicago Flow
Загружено: 2021-03-11
Просмотров: 17817
Описание:
How to decide between cells controls and beads controls.
Video Contents:
0:00 Intro
1:18 Unmix with all beads
2:09 Setting gates on beads in the unmixing wizard
5:56 Setting up worksheets to check unmixing
12:38 Determine which beads controls caused unmixing errors
23:39 Unmixing a second time with a combination of beads and cells
25:37 Setting gates on cells in the unmixing wizard
31:04 Assessing unmixing quality after using beads and cells
31:38 Troubleshooting unmixing errors
35:02 Final checks if there are no unmixing errors
36:00 Applying compensation to make final minor adjustments
39:33 Outro
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