Process is the Product: A New Analytical Tool for AAV Production Optimization and Monitoring

Chromatographic analysis of complex AAV presents challenges due to the presence of various impurities, including proteins, DNA, and other matrix species that may coelute with target molecule. In case where one-dimensional chromatography is insufficient for satisfactory separation, more efficient analytical tools are required to address these issues.

This webinar will explore the determination of percentage of full capsids in complex samples, including lysed harvests and spent media, with rapid analysis (results provided within 25 minutes per sample) and baseline separation of empty and full capsids.

Practical applications of the PATfix AAV Switcher will be highlighted, demonstrating its role in optimizing upstream processes through two rounds of DoE. Additionally, we will discuss how this analytical tool, alongside digital PCR (dPCR), helped narrow the harvesting time window, reducing accumulation of empty capsids. Finally, participants will gain insights into how the novel analytics can be integrated into their workflows to enhance efficiency and product quality.

What You Will Learn:
Gain insights into how the at-line analytics provides precise and reliable data that can inform critical decisions in bioprocessing, leading to more effective and efficient production strategies.
Discover the practical application of the at-line analytical monitoring in bioreactor runs, specifically in the context of AAV8 production in HEK suspension cells, and how it can be implemented into your existing processes to achieve better results.
Learn how leverage optimization of upstream processes through Design of - Experiments (DoE) by utilizing PATfix AAV Switcher Empty/Full capsid data.
Gain a comprehensive understanding of the PATfix AAV Switcher, including its capabilities and operational framework.

Speakers:
Blaž Bakalar, Product Manager PATfix® Platform, Sartorius BIA Separations
Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA Separations

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