Creating Bacterial Glycerol Stocks

Racheal, a Lab Tech here at Addgene, shows you how to create a glycerol stock solution to store your plasmids indefinitely.

For the full protocol text, visit http://www.addgene.org/protocols/crea...

Bacterial glycerol stocks are important for long-term storage of plasmids. The addition of glycerol stabilizes the frozen bacteria, preventing damage to the cell membranes and keeping the cells alive. A glycerol stock of bacteria can be stored stably at -80°C for many years.

This protocol begins after you’ve already created a bacterial culture, so please see out video on Inoculating Liquid Bacterial Culture.
http://www.addgene.org/protocols/inoc...
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0:00 Intro & Overview
1:22 Materials
2:27 Creating the Bacterial Glycerol Stocks
3:39 Recovering Bacteria from your Glycerol Stocks
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Featuring: Racheal Komuhendo
Written, Directed, & Edited by: Quintin Marcelino
Music by: Lauren Duski, Vibe Mountain, and RKVC