Mycomaterial Incubation (Growing your mycelium based material)

What’s one thing you’ve learned (or struggled with) during incubation? Let us know in the comments so we can troubleshoot together!

During incubation, environmental factors are carefully adjusted to support the growth of mycelium and prepare it for the next stage of development or application. In the context of myco-composites, the goal is to have the mycelium fully infuse the chosen substrate, creating a dense, cohesive and durable material. The importance of proper incubation really can’t be overstated because it lays the foundation for a strong material and avoids competing organisms from taking over your myceliated substrate. By being present and tending to these conditions, you’re giving your mycelium the best possible environment to grow.

Temperature:
The ideal incubation temperature is usually between 20 and 25°C (68–77°F) for most mushroom strains. Keep in mind that mycelium is metabolically active and can heat up the space it’s in, so checking the internal temperature regularly is a good idea to avoid overheating.

Fresh and clean air exchange:
Fresh air exchange is essential to prevent CO₂ buildup, which can slow mycelial growth and encourage mold or bacterial competition. But you need a balance, too much airflow can dry out the substrate. Aim for gentle, clean exchange and observe what your setup needs.

Humidity:
Relative humidity around 85 to 90% is usually ideal. It helps keep your substrate moist, which supports growth. Too much moisture, though, can create an opening for contamination, so avoid anything too soggy.

Clean environment:
Competing organisms (aka contamination) is the main thing to watch out for. Mold, bacteria and competing fungi can easily outcompete your mycelium. Clean tools, surfaces and hands are essential. Try not to open your incubation space unless necessary to reduce exposure.

Darkness:
Most mycelium prefers to grow in the dark. Light can trigger fruiting, so especially for material applications, it’s best to keep your mycelium in darkness until you’re ready for the next step.

Substrate nutrient balance:
The nutrients in your substrate play a big role. If it’s too low in nutrients, growth can be slow or weak. If it’s too rich, other fungi or molds might show up. Make sure your mix has enough structure and air pockets too – mycelium needs room to move.

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Stay entangled,

Jess & Cat