THIN-LAYER CHROMATOGRAPHY | DETECTION METHODS | UV/IODINE-CHAMBER METHOD | SPRAY REAGENT METHOD |
Автор: Chemistry Lessons
Загружено: 2025-07-20
Просмотров: 160
In this video, I will explain the following with suitable examples:
1. DETECTION METHODS in THIN-LAYER CHROMATOGRAPHY
a. Visualisation or Detection methods for colourless compounds: CHEMICAL & PHYSICAL METHODS
I. Chemical method (using visualising spray reagents): Destructive method (sample is destroyed before detection)
II. Physical methods (Iodine, UV chamber & Densitometric method): Non-destructive method (sample is not destroyed)
III. Densitometric method (in situ method)
2. Applications of the THIN-LAYER chromatographic separation method
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