GENE EDITING EXPLAINED! - A comprehensive guide to the principles, methods and technologies!

An “In Depth” lecture on gene editing brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.
https://www.gla.ac.uk/people/adamwest

Time stamps:
3:41 Introduction
9:04 DNA repair pathways
17:20 Possible genome editing outcomes using sequence-specific nucleases
23:32 Zinc finger nucleases (ZFNs)
34:38 Transcription activator-like effector nucleases (TALENs)
44:09 CRISPR adaptive immune systems
50:01 Using CRISPR/Cas9 for genome editing
1:10:54 CRISPR specificity
1:35:52 Precision editing
1:40:00 Homology directed repair
1:44:19 Enzymatic base editing
1:47:20 Prime editing

The presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.

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More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.

Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides.

Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.

Publication List

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6. Wolfe SA et al. Annu Rev Biophys Biomol Struct. 2000;29:183-212.
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8. Urnov FD et al. Nat Rev Genet. 2010 Sep;11(9):636-46.
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9. Carroll D. Genetics. 2011 Aug;188(4):773-82.
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10. Kim H and Kim JS. Nat Rev Genet. 2014 May;15(5):321-34.
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12. Boch J and Bonas U. Annu Rev Phytopathol. 2010;48:419-36.
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13. Bogdanove AJ et al. Curr Opin Plant Biol. 2010 Aug;13(4):394-401.
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15. Joung JK and Sander JD. Nat Rev Mol Cell Biol. 2013 Jan;14(1):49-55.
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16. Mussolino C and Cathomen T. Curr Opin Biotechnol. 2012 Oct;23(5):644-50.
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17. Mali P et al. Nat Methods. 2013 Oct;10(10):957-63.
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19. Garside EL and MacMillan AM. Nat Biotechnol. 2014 Apr;32(4):338-40.
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20. Nishimasu H et al. Cell. 2014 Feb 27;156(5):935-49.
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21. Hsu PD et al. Cell. 2014 Jun 5;157(6):1262-78.
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22. Hendel A et al. Trends Biotechnol. 2015 Feb;33(2):132-40.
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23. Liang X et al. J Biotechnol. 2015 Aug 20;208:44-53.
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24. Brinkman EK et al. Nucleic Acids Res. 2014 Dec 16;42(22):e168.
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25. Koo T et al. Mol Cells. 2015 Jun;38(6):475-81.
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30. Howden SE et al. Stem Cell Reports. 2016 Sep 13;7(3):508-517.
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31. Fu Y et al. Nat Biotechnol. 2014 Mar;32(3):279-284.
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32. Slaymaker IM et al. Science. 2016 Jan 1;351(6268):84-8.
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33. Kleinstiver BP et al. Nature. 2016 Jan 28;529(7587):490-5.
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34. Komor AC et al. Cell. 2017 Jan 12;168(1-2):20-36.
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37. Hess GT et al. Mol Cell. 2017 Oct 5;68(1):26-43.
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38. Gaudelli NM et al. Nature. 2017 Nov 23;551(7681):464-471.
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39. Anzalone AV et al. Nature. 2019 Dec;576(7785):149-157.
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Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(YouTube Audio Library)