Leica SP8 Tutorial

This video describes startup and shutdown as well as control of the Leica SP8 confocal via LasX software.

00:13 - Hardware startup
02:34 - Start LasX
03:25 - LAsX overview
04:04 - Using Dye Assistant
06:29 - xy scan parameters
07:50 - setting channel gains and lasers
10:19 - Using digital zoom
11:09 - Taking an xy scan
11:42 - Using averaging for scans
12:59 - Setting z stacks
15:20 - Using Z compensation
16:50 - Intro to 3D renderer
17:32 - Optimising system for speed using dye assistant
18:11 - Using LasX Navigator
19:14 - Setting multiple single image xyz scans
21:12 - Taking tilescan images with support focus points
23:49 - Using mosaic merge to fuse tilescans
24:45 - Saving scan settings
24:54 - Using HyD detectors in photon counting mode
26:21 - Lif Project file overview and saving your data
27:18 - Reusing Navigator to find areas to image in LasX
28:42 - Using 3D renderer to create images and movies
31:36 - Editing images in lif Projects and changing default image name
32:23 - Capturing Transmission images on the transmitted light detector
33:46 - saving snapshot RGB images
34:36 - Saving lif files and shutting down LasX
35:00 - Logout or Shutdown PC
35:23 - Hardware shutdown