Primer design for identifying mutations in a CRISPR experiment using CHOPCHOP

The Wermeling lab at the Karolinska Institutet
– Research related to Autoimmunity, Cancer immunotherapy and CRISPR/Cas9

Using experimental systems and patient material we study the immune system with a specific interest related to inflammation, autoimmune disease, and cancer immunotherapy.

A major part of the different projects in the lab relates to antibodies, neutrophils and T cells. We also spend a lot of effort into using CRISPR/Cas9 based custom screens as a discovery platform to understand complex biological processes and to identify novel drug targets. To facilitate the design of these screens, we have developed a software that we call Green Listed (Panda et al, 2017, Bioinformatics).