Lipidomics of retinoblastoma

Seetaramanjaneyulu Gundimeda, Ph.D.
Medgenome Labs Ltd.
Bengaluru | India

Part of the webinar
Metabolomics India 2025

Title
Lipidomics of retinoblastoma

Retinoblastoma (RB) is a rare ocular cancer seen in children that counts for approximately 3% of all childhood cancers. lt is found that mutation in RBl, a tumor Suppressor gene on chromosome 13 as the cause of malignancy. We studied lipidomics of two RB cell lines, one aggressive cell line (NCC-RbC-51) derived from a metastatic site and one non-aggressive cell line (WERI-Rbl) in
comparison with a control cell line (MIO-Ml). Lipid profiles of all the cell lines were studied using high resolution mass spectrometer coupled to high performance Liquid Chromatography. Data acquired from all the three cell lines in positive mode were analyzed to identify differentially expressed metabolites. Several phospholipids and lysophospholipids were found to be dysregulated. We observed upregulation of hexosyl ceramides, and down regulation of dihydroceramides and higher order sphingoglycolipids hinting at a hindered sphingolipid biosynthesis. The results obtained from Liquid Chromatography­Mass Spectrometry are validated by using qPCR and it was
observed that genes involved in ceramide biosynthesis pathway are getting down regulated.

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