How to Leverage Illumina Sequencing in Genome-Wide CRISPR Screens

Learn about how Clustered Regularly Interspaced Short Palindromic repeats (CRISPR) screens can be an important tool in your research, as well as how to perform a successful experiment using NGS as a downstream readout.

Presenter: Lauren Fait– Sr. Sequencing Specialist, Illumina

00:00 Introduction
00:32 CRISPR Screening Overview
2:28 Natural CRISPR Systems Have 2 Major Components
5:11 CRISPR Screen Overall Workflow
6:49 sgRNA Library Assembly and Packaging
9:04 NGS Quality Control for Pooled Libraries
10:05 Pooled CRISPR Screen
15:12 Genome-Scale Knockout Libraries
18:00 Hit Identification using Illumina Sequencing
20:58 Single-Cell Sequencing for CRISPR Screens

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A global genomics leader, Illumina provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Illumina technology is responsible for generating more than 90% of the world’s sequencing data.* Through collaborative innovation, Illumina is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond.

*Data calculations on file. Illumina, Inc., 2015.

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