Fiber-seq: removing barriers to long-read epigenomics

At PacBio PRISM Boston, Bryan Venters of EpiCypher discusses how his team is removing key barriers to Fiber-seq, a powerful long-read technology for multi-omic epigenomic analysis. Discover how PacBio's SPRQ chemistry and Revio system are helping make these advanced studies more accessible for the scientific community.

Fiber-seq uses the Hia5 enzyme to simultaneously map chromatin accessibility (6mA), nucleosome positions, and transcription factor footprints, all while preserving genetic variants and 5mC data on single, multi-kilobase molecules. Venters explains how EpiCypher is addressing challenges like the lack of commercial Hia5 and optimized protocols by producing the enzyme at scale and developing robust workflows.

This presentation details the rich data generated by Fiber-seq, from mapping nucleosome periodicity around promoters to identifying individual CTCF binding events with single-molecule resolution. Venters demonstrates how the method can infer RNA polymerase locations and even predict long-range genomic contacts, offering insights comparable to specialized assays like Hi-C but from a single, comprehensive experiment.

00:00 Introduction & Revio installation
01:50 The need for long-read epigenomics
04:33 How Fiber-seq works with Hia5 methyltransferase
06:25 EpiCypher's solution: commercializing Fiber-seq
08:28 Initial Fiber-seq data & protocol optimization
12:18 Mapping nucleosomes & transcription factors
17:33 Inferring Pol II and predicting 3D contacts
19:27 Summary & early access program

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