Gel Electrophoresis | Agarose Gel Electrophoresis Lab Procedure

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Gel electrophoresis is a method to separate biomolecules.. This property depends upon the shape and weight of the molecule to be separated. To separate different kind of molecules, different sorts of gels are prepared. Like, agarose gel to separate DNA and RNA molecules. Depending upon the size, the concentration of the gels are increased or decreased. Thus in order to resolve smaller DNA/RNA samples high concentration agarose gels are prepared. If you want to differentiate between two molecules which are bigger or smaller by very few units of nucleotides, the size of the gels and concentration are increased as in case of simple sequence repeat markers. Nucleotides, due to their negative charges (presence of phosphate) migrate from negative to positive charge.

Separation of protein molecules require sophisticated PAGE Gels (Polyacrylamide)

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