High-Dimensional Spectroscopy to Tackle Complexity in Bio-NMR | Prof. Jan Stanek | Session 75

During the 75th session of the Global NMR Discussion Meetings held on November 21st, 2023 via Zoom, Prof. Jan Stanek from the University of Warsaw in Poland, gave a talk on the topic "High-dimensional spectroscopy and other tools to tackle complexity in biological solid-state NMR". The recording serves as a tutorial.

Website: http://nmr.cent3.uw.edu.pl/stanek

Abstract:
Fast (sup. 60 kHz) magic-angle spinning provides high sensitivity and narrowed 1H linewidths. However, spectral analysis of large proteins are often severely hampered by peak overlap and/or ambiguity, depending on system size and sample quality. In this tutorial lecture, I will discuss several experimental techniques to address this, namely high-dimensional (4D, 5D) spectroscopy with non-uniform sampling, projection spectroscopy and time-shared acquisition. Additionally, I will explore selected tools for automation of resonance assignment and present early data on protein dynamics measurement as pseudo-4D series.

Current organizers:
Adrian Draney (Creighton Uni., Chemistry)
Amrit Venkatesh (Florida State University, National High Magnetic Field Laboratory)
Asif Equbal (New York Uni., Abu Dhabi)
Blake Wilson (Robert Tycko Lab, NIH)
Michael Hope (Warwick Uni., Chemistry)
Mouzhe Xie (Maurer Lab, Uni. Chicago)
Nino Wili (Niels Chr Nielsen Lab, Aarhus Uni.)
Tamar Wolf (Lucio Frydman Lab, Weizmann Institute of Science)
Nesreen Elathram (Debelouchina Lab, UCSD)
Charlotte Bocquelet (HMRLab, CRMN Lyon)