Writing A Plate Measurement Method With the AKTA Pure

Here we write a simple method to measure plates of a column in Unicorn for AKTA Pure Chromatography Systems. Plates are an excellent way to determine how good your column is.

Links to videos I talked about:
Introduction to chromatography plates:    • How good is your chromatography column?  
Understanding blocks in Unicorn:    • Understanding blocks in Unicorn software f...  
Reading Unicorn instructions:    • Reading Unicorn Language With AKTA Chromat...  
Making a custom column:    • Making a Custom Column in Unicorn Chromato...  
Synchronizing blocks:    • Synchronizing blocks in Unicorn Chromatogr...  

For further reading generally on how to use the AKTA Pure see: https://cdn.cytivalifesciences.com/ap...

For more reading on creating methods: https://cdn.cytivalifesciences.com/ap...

Please leave a comment below and good luck with your purifications!

Reach me at my Linkedin page   / chad-blamey-ph-d-649a336  

I don't work for Cytiva & I do not represent them in any way. The view's expressed here are my own.

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Here's the method for you to use if you want it.

Main method:
0.00 Base: CV, Vc=120.637 {ml}, HiLoad 16/60 Superdex 200 pg
0.00 Phase: Method Settings
0.00 Base: Volume, Any
0.00 Alarm pre column pressure: Enabled, 0.30 {MPa}, 0.00 {MPa}
0.00 Alarm delta column pressure: Enabled, 0.50 {MPa}, 0.00 {MPa}
0.00 Wavelength: 280 {nm}, Off {nm}, Off {nm}
0.00 Noise reduction UV: 0.2 {sec}
0.00 Injection valve: Manual load
0.00 Column position: By-pass, Down flow
0.00 Pump A wash: On
0.00 System flow: (1.000)#Flow rate {ml/min}, Pre column pressure
5.00 End_Block
0.00 Phase: Column Equilibration
0.00 Base: SameAsMain
0.00 Column position: (1)#Column Position, Down flow (1.00)#Length of equilibration End_Block
0.00 Phase: Salt Injection
0.00 Base: SameAsMain
0.00 Injection valve: Inject
0.02 Injection valve: Manual load
0.02 End_Block
0.00 Phase: Plate Run
0.00 Base: SameAsMain (1.50)#Length of Run End_Block
(1.50)#Length of Run End_Block


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