Column chromatography
Автор: Lab Life Tutorials
Загружено: 2018-11-01
Просмотров: 10133
In this video we explain and demonstrate how to set up a column for flash chromatography on silica, how to load the crude compound, and how to follow the separation of the product from the impurities.
Things to remember:
SAFETY:
1) Silica for column chromatography may cause irritation to the respiratory tract if inhaled. Handle it under a fume hood.
2) The organic solvents commonly used for column chromatography may be volatile and flammable. If that is the case, handle them under the fume hood and take all the necessary precautions.
GOLDEN RULE OF FLASH CHROMATOGRAPHY: never trash any fraction before you are 100% sure you have successfully isolated and collected your product.
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