How to Use Real-Time PCR to Study Plant Stress Responses | Gene Expression & Phenotyping Explained

Are you researching plant responses to stress and want to explore molecular phenotyping techniques? In this video, we break down how to use Real-Time PCR (qPCR) to monitor gene expression changes in plants under abiotic or biotic stress conditions. 🌱💥

We’ll cover:
✅ How stress affects plant phenotypes
✅ Selection of candidate genes for qPCR
✅ Sample preparation and RNA extraction
✅ cDNA synthesis and qPCR setup
✅ Data analysis (ΔΔCt method) to quantify gene expression
✅ Linking molecular data to observed phenotypes (e.g., wilting, leaf curling, ROS levels)

This tutorial is ideal for students, researchers, and plant biotechnologists who aim to correlate gene expression with phenotypic traits like drought tolerance, disease resistance, or salinity adaptation.

🔬 Unlock the power of molecular diagnostics in plant science today!

🔑 Keywords
Real-Time PCR in Plants

qPCR for Plant Stress

Gene Expression Profiling in Plants

Plant Phenotyping and qPCR

Molecular Plant Physiology

Abiotic Stress in Plants

Biotic Stress Gene Response

ΔΔCt Method Explained

cDNA Synthesis for Real-Time PCR

Plant Molecular Biology Tutorial

🏷️ Hashtags
#RealTimePCR #PlantStressResponse #qPCR #PlantBiotechnology #GeneExpression #MolecularPlantScience #Phenotyping #PlantPathology #AbioticStress #BioticStress #PCRtutorial #ΔΔCtMethod #PlantResearch