Detection: RNA-Binding Proteins By RNA Pull-Down In Adipocyte Culture l Protocol Preview
Автор: JoVE (Journal of Visualized Experiments)
Загружено: 2022-08-01
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Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture - a 2 minute Preview of the Experimental Protocol
Qianfan Bai, Zhiqiang Bai, Shaohai Xu, Lei Sun
Duke-NUS Medical School, Cardiovascular and Metabolic Disorders Program; Institute of Molecular and Cell Biology, Singapore,; Nanyang Technological University, Division of Bioengineering, School of Chemical & Biomedical Engineering;
An RNA pull-down protocol is optimized here for detection of interactions between RNA-binding proteins (RBPs) and noncoding as well as coding RNAs. An RNA fragment from androgen receptor (AR) was used as an example to demonstrate how to retrieve its RBP from lystate of primary brown adipocytes.
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