1 of 3 Which of the following is found in a crRNA (i.e., the processed CRISPR RNA) that is ready to…

1 of 3 Which of the following is found in a crRNA (i.e., the processed CRISPR RNA) that is ready to be used as a guide RNA to identify the target DNA or RNA? a) One full and part of another repeat sequence and one complete spacer sequence. b) One PAM sequence, one full repeat sequence, and one full spacer sequence. c) Two full repeat sequences and two complete spacer sequences. d) The leader sequence and two spacer sequences flanking each of the repeat sequences. 2 of 3 How do the 6S RNAs affect gene expression in E. coli cells growing at the stationary phase of growth? A) 6S RNAs bind Sigma S-RNAP complexes and help transcribe genes supposed to be activated during the stationary phase. B) 6S RNAs bind Sigma 70-RNAP holoenzyme complex and break up/degrade the holoenzyme. c) 6S RNAs bind Sigma 70-RNAP complexes and prevent Sigma 70-RNAP from binding gene promoters. 3 of 3 All of the following are functions of the cas proteins (or the cascade complex) in the CRISPR phenomenon of E. coli, EXCEPT a) processing the primary CRISPR RNA b) identifying and slicing the viral DNA or viral RNA. c) acquiring the spacer DNA from virus genomes d) transcribing the primary CRISPR RNA locus

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