Antimicrobial Activity and Cell Cytotoxicity Testing | Protocol Preview

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High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity - a 2 minute Preview of the Experimental Protocol

Lucius Chiaraviglio, Yoon-Suk Kang, James E. Kirby
Beth Israel Deaconess Medical Center, Department of Pathology;

A high throughput, real-time assay was developed to simultaneously identify (1) eukaryotic cell-penetrant antimicrobials targeting an intracellular bacterial pathogen, and (2) assess eukaryotic cell cytotoxicity. A variation on the same technology was thereafter combined with digital dispensing technology to enable facile, high-resolution, dose-response, and two- and three-dimensional synergy studies.

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