How to make a buffer 10X Stock Solution of TE Buffer, pH 8.0

Tris EDTA buffer is commonly used for nucleic acids to neutralize and stabilize the pH. Use to isolate, purify, and store DNA, cDNA, or RNA for cloning, qPCR, or gel electrophoresis. Here’s how to prepare a 10X TE stock solution that can easily be diluted 1:10 to make 1X TE buffer.

1. Weigh out 1.6g EDTA (0.01M) and 7.88g Tris-HCl or Trizma® Hydrochloride (0.1M).
2. Add 400 mL ultrapure Milli-Q ® H2O to a beaker with a stir bar.
3. Add Tris-HCl and EDTA, stirring until fully dissolved.
4. Use a calibrated pH meter to measure and adjust the buffer pH with HCl and NaOH to pH 8.0. Be sure to exercise caution when working with concentrated acids and bases.
5. Pour buffer into a volumetric flask and q.s. to a final volume of 500 mL with Milli-Q ® ultrapure H2O.
6. Mix well.
7. Label and store the 10X TE stock solution at room temperature or 4°C for 3 - 6 months.
8. Dilute 10X TE stock 1:10 to make fresh 1X TE working buffer for your assay. Also, you can autoclave or sterile filtered the buffer according to your lab and assay requirements.

EDTA: https://www.sigmaaldrich.com/US/en/pr...

Trizma® Hydrochloride: https://www.sigmaaldrich.com/US/en/pr...

HCl: https://www.sigmaaldrich.com/GB/en/pr...

NaOH: https://www.sigmaaldrich.com/GB/en/pr...

Milli-Q® Water Purification Systems: https://www.sigmaaldrich.com/GB/en/te...

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10X Tris EDTA: https://www.sigmaaldrich.com/GB/en/pr...