Dirty N95 Mask under the Microscope

Microscopic video footage of a clean and a dirty N95 mask under the microscope.

Cultures were grown on tryptic soy blood agar at 36 degrees Celsius for 48 hours.

Camera - Nikon D3300
Microscopes -
Leica ATC 2000
Wolfe Stereomicroscope (10x Magnification)

Microscope magnification of each shot is shown in the bottom right hand corner.


Music by Sci-inspi


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References

1. Larsen, Gregory S., Cheng, Yongqiang, Daemen, Luke L., Lamichhane, Tej N., Hensley, Dale K., Hong, Kunlun, Meyer, Harry M., Monaco, Steven J., Levine, Alan M., Lee, Richard J., Betters, Emma, Sitzlar, Kim, Heineman, Jesse, West, Justin, Lloyd, Peter, Kunc, Vlastimil, Love, Lonnie, Theodore, Merlin, and Paranthaman, Mariappan Parans. Polymer, Additives, and Processing Effects on N95 Filter Performance. United States: N. p., 2021. Web. doi:10.1021/acsapm.0c01294.

2. Adanur S, Jayswal A. Filtration mechanisms and manufacturing methods of face masks: An overview. Journal of Industrial Textiles. December 2020. doi:10.1177/1528083720980169

3. Taslim Ur Rashid, Sadia Sharmeen, and Shanta Biswas. Effectiveness of N95 Masks against SARS-CoV-2: Performance Efficiency, Concerns, and Future Directions. ACS Chemical Health & Safety 2022 29 (2), 135-164
DOI: 10.1021/acs.chas.1c00016

4. William G. Lindsley, Francoise M. Blachere, Brandon F. Law, Donald H. Beezhold & John D. Noti (2021) Efficacy of face masks, neck gaiters and face shields for reducing the expulsion of simulated cough-generated aerosols, Aerosol Science and Technology, 55:4, 449-457, DOI: 10.1080/02786826.2020.1862409

5.Leggett, Helen C et al. “Mechanisms of pathogenesis, infective dose and virulence in human parasites.” PLoS pathogens vol. 8,2 (2012): e1002512. doi:10.1371/journal.ppat.1002512

6.Brooks JT, Butler JC. Effectiveness of Mask Wearing to Control Community Spread of SARS-CoV-2. JAMA. 2021;325(10):998–999. doi:10.1001/jama.2021.1505

7.Yim, Wonjun et al. “KN95 and N95 Respirators Retain Filtration Efficiency despite a Loss of Dipole Charge during Decontamination.” ACS applied materials & interfaces vol. 12,49 (2020): 54473-54480. doi:10.1021/acsami.0c17333