Methods of Gene Transfer | Biotechnology | Class 12th Biology | NEET | CUET

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Different methods are available to achieve genetic transformation of plants i.e. the delivery of
the foreign DNA into the host plant. These are divided into two main groups
-
Indirect methods: In this case vector is needed for insertion of the foreign DNA into the
host genome.
example - Agrobacterium mediated gene transfer and virus mediated gene transfer.
-
Direct methods: This method is vector independent. The DNA is directly inserted into the
host genome.
example - Biolistic gene gun, liposome mediated gene transfer, microinjection, Heat shock method of gene transfer and electroporation method of gene transfer.